Info-enriched characterization of mobile populations. Morphological info from photos can incorporate towards the richness of flow cytometry details. One example is, the determine reveals an or else regular apoptosis assay making use of Annexin V and PI, including cell imaging to characterize cells in Every single population to expose morphologically unique features. These insights couldn't happen to be obtained from multiplex staining by yourself.
As samples are acquired about the Attune CytPix Flow Cytometer, the higher-velocity brightfield digital camera captures and retailers illustrations or photos of detected gatherings in a price around six,000 photographs for each second, according to the flow rate and graphic sizing. For higher flexibility, Attune Cytometric Software package helps you to modify the graphic seize frequency as desired and select a specific gate for image capture, if essential.
Blend acoustic focusing know-how with substantial-parameter Investigation for adaptable spectral unmixing and traditional workflows.
Find out new insights into your experimental data by visualizing populations working with combined fluorescence and morphology facts. Our software package interface wizards allow downsampling, concatenation (merged files) and dimensionality applications that happen to be created into your program for an intuitive, streamlined info Investigation encounter.
"An image is not really truly worth a thousand dots, but it surely really really helps to see pictures. It is excellent to determine pictures that correlate with staining. Such as, AnnexinV-positive cells which might be morphologically distinct than healthy cells, or CD14+ monocytes that are greater plus more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is illuminating to see how many doublets slip through standard singlet gates, and excellent to verify the standing of unpredicted double-favourable situations in my Investigation."
Pair your Attune flow cytometer using an autosampler for increased workflow performance, and insert robotic integration for optimum throughput.
Constant image good quality even at superior throughput Acoustic focusing plus a superior-speed digicam Blend to impression these T cells regularly at lower or high flow rates. Effortlessly modify concentrate and digicam settings to meet experimental specifications.
Early indications of this contamination had originally been dismissed as particles. Backgating to find out new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also enables you to use morphological characteristics to discover intriguing subpopulations that might not be obvious from flow cytometry data on your own.
Users can see event illustrations or photos in real-time to substantiate sample excellent, optimize protocols, and document the morphology of every mobile inhabitants recognized for Evaluation. Imaging capture also Gains from acoustic focusing, which helps you to situation the cells making sure that a sharp, centered image is received.
AI-pushed automated picture Examination With all the Attune Cytometric Program interprets occasion characteristics into distinct morphology parameters that can be combined with conventional fluorescence and scatter parameters website to reveal very reproducible populace improvements and exceptional subsets.
We Earlier shown the strength of imaging Auto-T/Ramos cell interactions. Permit’s check out just the inhabitants of finest desire, the double positive activities, To find out more. We can now use prolonged morphological parameters (here, circularity and skewness of depth) to further more study the features of such populations and refine gating on these occasions, escalating facts robustness.
Built-in dimensionality reduction resources elevated resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to confirm expected inhabitants resolution in lysed entire human blood. Consumer can check out activities from Every single cluster for Visible affirmation of cell phenotypes.
Adaptable payment program from Agilent Economic Alternatives allow you to get the important instrumentation you require for fast project, or to mature your lab.
Gating on these extended parameters means that you can rapidly and properly establish populations of interest and confirm gating technique with little if any manual critique. The Attune program provides use of condition-of-the-artwork equipment Studying Evaluation instruments within an expertly created person interface, which makes it exceptionally simple to operate.
The Attune Xenith cytometer is actually a higher-parameter spectral analyzer, supplying the two spectral unmixing and standard flow abilities for in depth and multipurpose mobile Examination.
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Listed here, we exhibit that by gating methods applying picture-based mostly quantitative parameters can distinguish interacting cells from coincident functions to more accurately examine interacting cells.